Aflatoxin M Family Immunoaffinity Column (AFM-IAC) Instruction Manual

Aflatoxin M family immunoaffinity column ( AFM-IAC )

1. Product introduction

Aflatoxins M1 (AFM1) and M2 (AFM2) are carcinogenic and mutagenic substances, which belong to one of aflatoxins and pose a great threat to human health. Therefore, aflatoxins in milk and dairy products in various countries. The M group content has strict limits.

This product cross-links specific monoclonal antibodies against aflatoxin M1 (AFM1) and M2 (AFM2) on agarose gel microspheres. Based on antigen-antibody specific binding, the sample is purified and aflatoxin is used. M1 (AFM1) and M2 (AFM2) specifically bind to the immunoaffinity column, and other substances flow out, and then rinse, elute, collect, and test, and complete the aflatoxins M1 (AFM1) and M2 in the sample. Determination of AFM2).

The efficiency, recovery rate and maximum load of this product are in line with national standards:

GB 5009.24-2016 (Determination of aflatoxin M family in foods for national food safety standards)

GB 5413.37-2010 (Determination of aflatoxin M1 in milk and dairy products)

2. Use

Suitable for aflatoxins in milk, milk powder, and low fat milk, skim milk, low fat milk powder, skim milk powder, etc.

M1 (AFM1), M2 (AFM2) content determination; purification, extraction and the like of aflatoxin M1 (AFM1) and M2 (AFM2) in the sample.

3. Product performance

4. Operation process

1 Sample treatment: The samples were treated according to the respective methods in GB 5009.24-2016 (Determination of aflatoxin M family in food safety national standards). 2 Purification: Take GB 5009.24-2016 (Determination of aflatoxin M in food safety national food) as an example (1) Column pretreatment: remove the aflatoxin M family immunoaffinity column and return to room temperature (22- At 25 ° C), remove the upper end plug, open the lower end plug, and drain the preservation solution in the column to 3-5 mm above the interface on the gel to stop the flow (or connect the immunochromatographic column directly to the 50 ml syringe).

(2) Adding sample: Add the total volume of 50ml of treated sample to the column, open the lower end plug, adjust the flow rate to 1-2 drops / sec, and discard the effluent. (3) Rinse: After the liquid is completely drained, add 10ml of distilled water and rinse (if necessary, increase the number of rinses).

(4) Blow dry: Use vacuum equipment (such as suction balls, syringes, etc.), press in the air, and dry the water in the column.

(5) Elution: Add 4 ml of acetonitrile (chromatographically pure), adjust the flow rate to 2-3 seconds per drop, slowly elute, elute for not less than 60 s, and collect all the eluents. (6) Treatment: Evaporate the eluent to 50-500 ul (not to dry) with gentle nitrogen at 30 ° C, then dilute with water.

10 to the final volume of 50-5000 ul, which is the purified sample.

3 detection

The purified sample is detected by mass spectrometry, high performance liquid phase, fluorescence, and the like.

5. Precautions

1 Aflatoxin is a carcinogen and should be avoided in direct contact with the body. Please wear gloves, masks, lab coats, etc. when handling. 2 The glass container that has been used in contact with the experiment should be soaked overnight with 5% sodium hypochlorite solution to avoid contamination.