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The aspartate aminotransferase (AST) assay kit is intended for the accurate and direct quantitative measurement of AST activity in human plasma. AST measurement is useful in the diagnosis of liver disease and heart disease.
Test principle
In the coupling reaction above, the oxidation rate of NADH is proportional to the activity of AST. The unit activity of AST can be calculated from the decreasing rate of absorbance detected at a wavelength of 340 nm. Â
Product Features
Â
Linear deviation ≤ ± 10.00%
Technical Parameters
Â
Temperature
37ºC
Optical path length (OPL)
1.0 cm
Main wavelength
340 nm
Secondary wavelength
450 nm (Optional)
Method
Velocity method
Reaction direction
Negative
Sample
12 μL
R1
200 μL
R2
50 μL
Delay time
90 s
Reaction time
250 s
Â
Â
Packing Specifications
R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
Â
The aspartate aminotransferase (AST) assay kit is intended for the accurate and direct quantitative measurement of AST activity in human plasma. AST measurement is useful in the diagnosis of liver disease and heart disease.
Test principle
In the coupling reaction above, the oxidation rate of NADH is proportional to the activity of AST. The unit activity of AST can be calculated from the decreasing rate of absorbance detected at a wavelength of 340 nm. Â
Product Features
Â
Linear deviation ≤ ± 10.00%
Technical Parameters
Â
Temperature
37ºC
Optical path length (OPL)
1.0 cm
Main wavelength
340 nm
Secondary wavelength
450 nm (Optional)
Method
Velocity method
Reaction direction
Negative
Sample
12 μL
R1
200 μL
R2
50 μL
Delay time
90 s
Reaction time
250 s
Â
Â
Packing Specifications
R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
Â
Model NO.: AST
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*40 mL, R2: 1*20 mL
Origin: Jinan, Shandong
Model NO.: AST
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*40 mL, R2: 1*20 mL
Origin: Jinan, Shandong
1. Linear range of ASTÂ activity: [3, 500] U/L
Linear correlation coefficient ∣r∣≥ 0.990
2. Absorbance of blank ≥ 0.90
3. Changing rate of absorbance of blank ΔA/min ≤ 0.02 Â
4. Sensitivity
Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific AST sample of activity of 109 U/L.5. Accuracy
Relative deviation is within ±10%.6. Precision
Intra CV% ≤ 6.0%; inter CV% ≤8.0%.1. Linear range of AST activity: [3, 500] U/L
Linear correlation coefficient ∣r∣≥ 0.990
2. Absorbance of blank ≥ 0.90
3. Changing rate of absorbance of blank ΔA/min ≤ 0.02 Â
4. Sensitivity
Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific AST sample of activity of 109 U/L.5. Accuracy
Relative deviation is within ±10%.6. Precision
Intra CV% ≤ 6.0%; inter CV% ≤8.0%.