Model NO.: AST
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*40 mL, R2: 1*20 mL
Origin: Jinan, Shandong
Model NO.: AST
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*40 mL, R2: 1*20 mL
Origin: Jinan, Shandong

The aspartate aminotransferase (AST) assay kit is intended for the accurate and direct quantitative measurement of AST activity in human plasma. AST measurement is useful in the diagnosis of liver disease and heart disease.

Test principle

Aspartate Aminotransferase (AST) Assay Kit/Test Kit/Ivd Reagents

In the coupling reaction above, the oxidation rate of NADH is proportional to the activity of AST. The unit activity of AST can be calculated from the decreasing rate of absorbance detected at a wavelength of 340 nm.  

Product Features
 
1. Linear range of AST activity: [3, 500] U/L
Linear correlation coefficient ∣r∣≥ 0.990
Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≥ 0.90
3. Changing rate of absorbance of blank ΔA/min ≤ 0.02  
4. Sensitivity
Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific AST sample of activity of 109 U/L.
5. Accuracy
Relative deviation is within ±10%.
6. Precision
Intra CV% ≤ 6.0%; inter CV% ≤8.0%.

Technical Parameters
 
Temperature 37ºC Optical path length (OPL) 1.0 cm
Main wavelength 340 nm Secondary wavelength 450 nm (Optional)
Method Velocity method Reaction direction Negative
Sample 12 μL R1 200 μL
R2 50 μL Delay time 90 s
Reaction time 250 s    
Reference range of AST activity: 11 - 34 U/L.

Packing Specifications

R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)

Storage and stability

Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
 
The aspartate aminotransferase (AST) assay kit is intended for the accurate and direct quantitative measurement of AST activity in human plasma. AST measurement is useful in the diagnosis of liver disease and heart disease.

Test principle

Aspartate Aminotransferase (AST) Assay Kit/Test Kit/Ivd Reagents

In the coupling reaction above, the oxidation rate of NADH is proportional to the activity of AST. The unit activity of AST can be calculated from the decreasing rate of absorbance detected at a wavelength of 340 nm.  

Product Features
 
1. Linear range of AST activity: [3, 500] U/L
Linear correlation coefficient ∣r∣≥ 0.990
Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≥ 0.90
3. Changing rate of absorbance of blank ΔA/min ≤ 0.02  
4. Sensitivity
Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific AST sample of activity of 109 U/L.
5. Accuracy
Relative deviation is within ±10%.
6. Precision
Intra CV% ≤ 6.0%; inter CV% ≤8.0%.

Technical Parameters
 
Temperature 37ºC Optical path length (OPL) 1.0 cm
Main wavelength 340 nm Secondary wavelength 450 nm (Optional)
Method Velocity method Reaction direction Negative
Sample 12 μL R1 200 μL
R2 50 μL Delay time 90 s
Reaction time 250 s    
Reference range of AST activity: 11 - 34 U/L.

Packing Specifications

R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)

Storage and stability

Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
 

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