Puyang Linshi Medical Supplies Co., Ltd. , https://www.linshimedicals.com
The ovulatory test paper is a test of the peak levels of the luteinized hormone (LH) to predict ovulation.
Can be used for qualitative detection of urine luteinizing hormone (LH), so as to determine the time of ovulation and "rhythm" in women's menstrual cycle and to choose the best time to conception or use "safe period" the purpose of contraception.
Ovulation test paper is an in vitro examination and auxiliary diagnosis of ovulation.
Ovulatory test paper is suitable for the use of about 30% of females, in clinical use only as the auxiliary means to detect ovulation.
The principle
This reagent with double antibody immune chromatography method principle and colloidal gold marked technology, is composed of solid phase respectively has the a - LH in rat monoclonal antibody (line) and sheep polyclonal antibody against rat IgG nitrocellulose membrane (control), adsorption with colloidal gold labeling in advance rat beta LH monoclonal antibody resistance of glass fibers and other auxiliary materials paste made in turn.
Detection, LH in urine sample and test sample pad side in advance of the solid phase beta LH monoclonal antibody colloidal gold combined into, the combination with the capillary action of membrane upward migration to test line (T), and a - on the membranes of the LH antibody reacting, appear a red stripe, whether in the specimen is LH, when the liquid level continue to migrate to control line (C), the region will appear a red stripe.
The color of the red stripe in the detection line (T) can be darker or similar if the color of the red stripe is darker than that of the control line (C), and the positive results indicate that the tested person is in ovulation period.
The color of the red stripe in the detection line (T) is less than the color of the red stripe in the control line area (C), and the negative result indicates that the tested person is not in ovulation period.
The red stripe shown in the control line (C) is the standard for determining whether there are enough specimens and whether the process of chromatography is normal, as well as the internal control standard for reagents.
We according to the principle of oviposit test paper to find out the most suitable method to detect ovulation ovulation oviposit test paper, it is in the period after the end of the week, the ovulation test were used to detect once a day, if it is found that oviposit test paper up strong Yang gradually enhanced, to strengthen the frequency of testing, it is better to test every 4 hours at a time.
If you compare the test paper, the ovulation paper starts to weaken, and it weakens quickly, indicating that the ovulation is about to be ovulating, so you can arrange the house.
The time range of ovulation is usually within 24 to 48 hours after ovulation.
Intended use
The reagents used for the qualitative detection in vitro women urine luteinizing hormone generated content changes, so as to determine the time of ovulation and "rhythm" in women's menstrual cycle and to choose the best time to conception or use "safe period" the purpose of contraception.
Women with normal ovulation draw each month, a mature eggs in the ovaries, after discharge from the ovary, through the fallopian tube to the uterus, pregnancy is through a mature egg in the fallopian tube combined with sperm.
Sperm in female reproductive tract about 24-72 hours can survive, while a fertilized egg suitable time only about 24 to 36 hours after ovulation, so 2 to 3 days before ovulation and 1 to 2 days after ovulation is vulnerable to pregnancy, or has a total of 4-5 days after ovulation is vulnerable to pregnancy, this paper can reliably detect the LH peak, her performance to predict the best time to conception or birth control.
Sample request
1 the reagent used human urine as a test specimen.
Use clean, dry containers to collect urine, urine can be detected in a day, but not usually morning urine.
Urine collection period after the end of a week is the best time of 10 am to 8 PM, in the process of consecutive days of testing, should try to use test those days every day at the same time of the urine samples as well.
3. If the urine appears to be cloudy, it should be centrifuged, filtered or treated with its precipitate before taking the upper cleaning liquid.
If not timely detection, urine samples can be stored at room temperature for 4 hours or in 48 hours 2-8 ºC refrigerated storage, long-term preservation should be frozen in - 20 ºC, avoid repeated freezing and thawing.
The addition of 0.1% sodium azide as protectant did not affect the test results.
5. Reduce water intake in the first 2 months of urine collection, since the diluted urine sample will interfere with the detection of the peak of LH.
[1]
Method of operation
1. The normal normal menstrual cycle is 28 days. The next period of menstruation is 14 days for ovulation days, generally starting from the 11th day of ovulation day, and it has been tested for 6 days.
But not every woman was ovulating in the middle of the menstrual cycle, and no positive results were found during the six-day period.
Some women are sometimes affected by the environment, mood and fatigue, and may ovulate in advance.
2. Water intake should be reduced 2 hours before urine collection, as diluted urine samples will interfere with the detection of the peak of LH.
3. Changes in the chromaticity of the test line should be observed and recorded. If the test results are negative, the chromaticity of the test line will start to decrease and can be used as the peak of LH.
4. The test paper should observe the reaction results within the stipulated time and will not be valid after 30 minutes.
With negative test results, the location of the detection line may appear a shallow ribbon, and then observe after a period of time.
5. Do not allow the test bar to be damp or touch reaction film before use, please immediately cover the tank lid immediately after the test bar is taken out.
Test strips should be used within the validity period. Do not use them after expiration.
6. HCG in the urine will interfere with the test results of the test paper. Therefore, ovulation test paper is not suitable for pregnant women. If there is a peak of LH in the last few days, the pregnancy should be tested first.
7. Currently, it is not seen that general common drugs (such as cold medicine, antibiotics, painkillers, etc.) will affect the accuracy of the test, but the injection or taking of drugs containing HCG will affect the test.
[2]
Inspection methods
The tested samples, reagents and other equipment should be restored at room temperature.
1. Before testing
- be sure to read the instructions on the packaging and instructions carefully before using them.
- before you use it, you need to know your regular menstrual cycle to ensure that you have a surge of LH at the right time.
- the total number of days from the first day of the menstrual cycle (the first day of the entire period) to the next menstrual period is the length of your menstrual cycle.
- after knowing the length of the menstrual cycle, use the following table to find when to start the test:
Length of menstrual cycle (days) 21 or less 22 23 24 25 26 27 28 29 30 31 32 33 34 35 38 38 39 40 41 or longer
Counting from the first day menstruation, and according to your menstrual cycle length below correspond to the number of days starting test 5 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 17 days before the start of the next menstrual period
- you can test at any time of the day, but the daily test time should be the same.
- try not to urinate during the first four hours of the test and avoid excessive fluid intake before testing. You will find it more convenient to use the morning urine test on the day.
2. Readiness test
Combine the atlas - remove the ovulation test pen from the aluminum foil packaging when preparing the test and remove the white cap.
Take out immediately.
- simply place the suction rod down in the urine stream for 5 seconds.
- if you like, you can also collect urine samples with a clean, dry container and then immerse the absorbent sticks in your urine for 20 seconds.
3. Wait 3 minutes - cover with a white cap, keep the suction rod down or put the egg test pen flat.
The test results will be displayed in 3 minutes.
4. Read test results
- to read the test results, check the results window for the blue line, which is the surge line and the quality control line.
- the surge line is closest to the arrow:
If the color of the line or surge line is less than the color of the quality control line, you have not detected a surge in LH.
Please retest at the same time tomorrow.
If the sharp line color is as deep as the quality control line or is deeper than the quality control line, you have detected a surge in LH, which is the easy conception time in the menstrual cycle.
In a 48-hour pregnancy, your chances of getting pregnant will be greatly increased.
You do not need to continue testing in this cycle.
- the quality control line also indicates whether the test is correct *.
* if the quality control line is not displayed in 10 minutes, a new test pen should be used for retesting.
Check the results in 10 minutes, and any changes in the window are ignored after this time.
Negative result
Only a red stripe or detection line appears in the control line.
The appearance of a red stripe, that is, the control line shows the color, the detection line does not display color, the expression is not ovulation;
Two red strips appear, and the detection line is noticeably lighter than the control line, indicating that the urine has not yet peaked and must be tested every day.
A positive result
Two colored strips appear and the detection line is equal to or deeper than the contrast line.
Indicates that the peak of LH has appeared, indicating that ovulation will occur within 24 to 48 hours.
Invalid results
The failure of the test failure or reagent failure occurred in the control line.
In this case, you should read the instructions carefully and retest them with the new reagent bar.
If the problem persists, you should immediately stop using the batch number and contact the local distributor and/or manufacturer.
considerations
1. The reagent is only used for external testing.
The reagent only opens the sealed aluminum foil bag before testing, and should be used as soon as possible after opening the original packaging.
3. Not every woman is ovulating in the middle of the menstrual cycle, and there may be no positive results during the 5 days of testing.
4. The chromaticity of the test line during the test should be observed and recorded. If the test result is negative, the chromaticity of the test line will start to decrease, and it can be used as the peak of LH.
5. The concentration of LH in the urine is too low, and the LH concentration in the urine is not representative. Therefore, too much water or other drinks should not be drunk before testing.
6 the test paper shall observe the reaction result within the specified time, and the result will be invalid after 30 minutes.
Do not allow the test bar to be damp or touch reaction film before use. Please immediately cover the tank cover immediately after the test bar is removed.
8 should be used within the validity period. Do not use after expiration.
9. Read the instructions carefully before starting the test, and do exactly according to each step;
Don't take drugs such as hormones that may affect the test results. When you avoid interfering with the test results, the pregnancy test will be accurate.
[3]
Issues related to
1 this reagent is used to test the urine specimen of human urine.
2 normal human homologous hormone hTSH article, hFSH try without interference, but urine HCG article try may interfere with the test results, this article try to not suitable for pregnant women, if it is found that the last few days in LH peak as a result, should first check whether pregnant.
3 general common drugs (such as cold medicine, antibiotics, analgesics, etc.) have not been reported to affect the accuracy of the test, but if injected or taken with a progesterone such as HCG, it can be affected.
When used for conception, ovulation is a complicated physiological process. Sometimes, the occurrence of the peak of LH does not necessarily result in the formation of normal yellow body after ovulation.
If the peak of LH is determined and the same room is not pregnant after 3 months, the gynecologist must be consulted.
When the test line is slightly lighter than the control line when used in the test, it is better to take contraceptive measures in the same room.
But women are sometimes affected by the environment, emotions and fatigue, and may be ovulating early.
The following may affect the test results: menopause, taking hormones, steroids and contraceptives, or polycystic ovary syndrome, hyperthyroidism and some endocrine diseases.
6 patients with primary hypogonadism, ovarian failure, and polycystic ovary syndrome can lead to abnormal increase of LH.
Pituitary - hypothalamus, amenorrhea syndrome, Kallma syndrome, anorexia nervosa.
The lack of simple LH in pituitary, and the delay of puberty can lead to abnormal decrease of LH, so the test results should be judged in a comprehensive clinical way.
7 oviposit test paper test is not absolutely accurate, miss ovulation and LH peak of abundant clinical case, many women can only detect the weak positive (representing the ovulation or had ovulation) and detect strong positive is ovulation (representative), that's not saying no ovulation.
Pregnant mothers can also go to the house during the weak positive period [4], and the chance of successful conception is not low.
Please use under the guidance of the doctor.
In this paper, the principle of double antibody sandwich and the technique of colloidal gold labeling are adopted in the paper, which is of high accuracy in determining the time of ovulation and the "safe period" in the menstrual cycle of women.
Â
The ovulatory test paper is a test of the peak levels of the luteinized hormone (LH) to predict ovulation.
Can be used for qualitative detection of urine luteinizing hormone (LH), so as to determine the time of ovulation and "rhythm" in women's menstrual cycle and to choose the best time to conception or use "safe period" the purpose of contraception.
Ovulation test paper is an in vitro examination and auxiliary diagnosis of ovulation.
Ovulatory test paper is suitable for the use of about 30% of females, in clinical use only as the auxiliary means to detect ovulation.
The principle
This reagent with double antibody immune chromatography method principle and colloidal gold marked technology, is composed of solid phase respectively has the a - LH in rat monoclonal antibody (line) and sheep polyclonal antibody against rat IgG nitrocellulose membrane (control), adsorption with colloidal gold labeling in advance rat beta LH monoclonal antibody resistance of glass fibers and other auxiliary materials paste made in turn.
Detection, LH in urine sample and test sample pad side in advance of the solid phase beta LH monoclonal antibody colloidal gold combined into, the combination with the capillary action of membrane upward migration to test line (T), and a - on the membranes of the LH antibody reacting, appear a red stripe, whether in the specimen is LH, when the liquid level continue to migrate to control line (C), the region will appear a red stripe.
The color of the red stripe in the detection line (T) can be darker or similar if the color of the red stripe is darker than that of the control line (C), and the positive results indicate that the tested person is in ovulation period.
The color of the red stripe in the detection line (T) is less than the color of the red stripe in the control line area (C), and the negative result indicates that the tested person is not in ovulation period.
The red stripe shown in the control line (C) is the standard for determining whether there are enough specimens and whether the process of chromatography is normal, as well as the internal control standard for reagents.
We according to the principle of oviposit test paper to find out the most suitable method to detect ovulation ovulation oviposit test paper, it is in the period after the end of the week, the ovulation test were used to detect once a day, if it is found that oviposit test paper up strong Yang gradually enhanced, to strengthen the frequency of testing, it is better to test every 4 hours at a time.
If you compare the test paper, the ovulation paper starts to weaken, and it weakens quickly, indicating that the ovulation is about to be ovulating, so you can arrange the house.
The time range of ovulation is usually within 24 to 48 hours after ovulation.
Intended use
The reagents used for the qualitative detection in vitro women urine luteinizing hormone generated content changes, so as to determine the time of ovulation and "rhythm" in women's menstrual cycle and to choose the best time to conception or use "safe period" the purpose of contraception.
Women with normal ovulation draw each month, a mature eggs in the ovaries, after discharge from the ovary, through the fallopian tube to the uterus, pregnancy is through a mature egg in the fallopian tube combined with sperm.
Sperm in female reproductive tract about 24-72 hours can survive, while a fertilized egg suitable time only about 24 to 36 hours after ovulation, so 2 to 3 days before ovulation and 1 to 2 days after ovulation is vulnerable to pregnancy, or has a total of 4-5 days after ovulation is vulnerable to pregnancy, this paper can reliably detect the LH peak, her performance to predict the best time to conception or birth control.
Sample request
1 the reagent used human urine as a test specimen.
Use clean, dry containers to collect urine, urine can be detected in a day, but not usually morning urine.
Urine collection period after the end of a week is the best time of 10 am to 8 PM, in the process of consecutive days of testing, should try to use test those days every day at the same time of the urine samples as well.
3. If the urine appears to be cloudy, it should be centrifuged, filtered or treated with its precipitate before taking the upper cleaning liquid.
If not timely detection, urine samples can be stored at room temperature for 4 hours or in 48 hours 2-8 ºC refrigerated storage, long-term preservation should be frozen in - 20 ºC, avoid repeated freezing and thawing.
The addition of 0.1% sodium azide as protectant did not affect the test results.
5. Reduce water intake in the first 2 months of urine collection, since the diluted urine sample will interfere with the detection of the peak of LH.
[1]
Method of operation
1. The normal normal menstrual cycle is 28 days. The next period of menstruation is 14 days for ovulation days, generally starting from the 11th day of ovulation day, and it has been tested for 6 days.
But not every woman was ovulating in the middle of the menstrual cycle, and no positive results were found during the six-day period.
Some women are sometimes affected by the environment, mood and fatigue, and may ovulate in advance.
2. Water intake should be reduced 2 hours before urine collection, as diluted urine samples will interfere with the detection of the peak of LH.
3. Changes in the chromaticity of the test line should be observed and recorded. If the test results are negative, the chromaticity of the test line will start to decrease and can be used as the peak of LH.
4. The test paper should observe the reaction results within the stipulated time and will not be valid after 30 minutes.
With negative test results, the location of the detection line may appear a shallow ribbon, and then observe after a period of time.
5. Do not allow the test bar to be damp or touch reaction film before use, please immediately cover the tank lid immediately after the test bar is taken out.
Test strips should be used within the validity period. Do not use them after expiration.
6. HCG in the urine will interfere with the test results of the test paper. Therefore, ovulation test paper is not suitable for pregnant women. If there is a peak of LH in the last few days, the pregnancy should be tested first.
7. Currently, it is not seen that general common drugs (such as cold medicine, antibiotics, painkillers, etc.) will affect the accuracy of the test, but the injection or taking of drugs containing HCG will affect the test.
[2]
Inspection methods
The tested samples, reagents and other equipment should be restored at room temperature.
1. Before testing
- be sure to read the instructions on the packaging and instructions carefully before using them.
- before you use it, you need to know your regular menstrual cycle to ensure that you have a surge of LH at the right time.
- the total number of days from the first day of the menstrual cycle (the first day of the entire period) to the next menstrual period is the length of your menstrual cycle.
- after knowing the length of the menstrual cycle, use the following table to find when to start the test:
Length of menstrual cycle (days) 21 or less 22 23 24 25 26 27 28 29 30 31 32 33 34 35 38 38 39 40 41 or longer
Counting from the first day menstruation, and according to your menstrual cycle length below correspond to the number of days starting test 5 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 17 days before the start of the next menstrual period
- you can test at any time of the day, but the daily test time should be the same.
- try not to urinate during the first four hours of the test and avoid excessive fluid intake before testing. You will find it more convenient to use the morning urine test on the day.
2. Readiness test
Combine the atlas - remove the ovulation test pen from the aluminum foil packaging when preparing the test and remove the white cap.
Take out immediately.
- simply place the suction rod down in the urine stream for 5 seconds.
- if you like, you can also collect urine samples with a clean, dry container and then immerse the absorbent sticks in your urine for 20 seconds.
3. Wait 3 minutes - cover with a white cap, keep the suction rod down or put the egg test pen flat.
The test results will be displayed in 3 minutes.
4. Read test results
- to read the test results, check the results window for the blue line, which is the surge line and the quality control line.
- the surge line is closest to the arrow:
If the color of the line or surge line is less than the color of the quality control line, you have not detected a surge in LH.
Please retest at the same time tomorrow.
If the sharp line color is as deep as the quality control line or is deeper than the quality control line, you have detected a surge in LH, which is the easy conception time in the menstrual cycle.
In a 48-hour pregnancy, your chances of getting pregnant will be greatly increased.
You do not need to continue testing in this cycle.
- the quality control line also indicates whether the test is correct *.
* if the quality control line is not displayed in 10 minutes, a new test pen should be used for retesting.
Check the results in 10 minutes, and any changes in the window are ignored after this time.
Negative result
Only a red stripe or detection line appears in the control line.
The appearance of a red stripe, that is, the control line shows the color, the detection line does not display color, the expression is not ovulation;
Two red strips appear, and the detection line is noticeably lighter than the control line, indicating that the urine has not yet peaked and must be tested every day.
A positive result
Two colored strips appear and the detection line is equal to or deeper than the contrast line.
Indicates that the peak of LH has appeared, indicating that ovulation will occur within 24 to 48 hours.
Invalid results
The failure of the test failure or reagent failure occurred in the control line.
In this case, you should read the instructions carefully and retest them with the new reagent bar.
If the problem persists, you should immediately stop using the batch number and contact the local distributor and/or manufacturer.
considerations
1. The reagent is only used for external testing.
The reagent only opens the sealed aluminum foil bag before testing, and should be used as soon as possible after opening the original packaging.
3. Not every woman is ovulating in the middle of the menstrual cycle, and there may be no positive results during the 5 days of testing.
4. The chromaticity of the test line during the test should be observed and recorded. If the test result is negative, the chromaticity of the test line will start to decrease, and it can be used as the peak of LH.
5. The concentration of LH in the urine is too low, and the LH concentration in the urine is not representative. Therefore, too much water or other drinks should not be drunk before testing.
6 the test paper shall observe the reaction result within the specified time, and the result will be invalid after 30 minutes.
Do not allow the test bar to be damp or touch reaction film before use. Please immediately cover the tank cover immediately after the test bar is removed.
8 should be used within the validity period. Do not use after expiration.
9. Read the instructions carefully before starting the test, and do exactly according to each step;
Don't take drugs such as hormones that may affect the test results. When you avoid interfering with the test results, the pregnancy test will be accurate.
[3]
Issues related to
1 this reagent is used to test the urine specimen of human urine.
2 normal human homologous hormone hTSH article, hFSH try without interference, but urine HCG article try may interfere with the test results, this article try to not suitable for pregnant women, if it is found that the last few days in LH peak as a result, should first check whether pregnant.
3 general common drugs (such as cold medicine, antibiotics, analgesics, etc.) have not been reported to affect the accuracy of the test, but if injected or taken with a progesterone such as HCG, it can be affected.
When used for conception, ovulation is a complicated physiological process. Sometimes, the occurrence of the peak of LH does not necessarily result in the formation of normal yellow body after ovulation.
If the peak of LH is determined and the same room is not pregnant after 3 months, the gynecologist must be consulted.
When the test line is slightly lighter than the control line when used in the test, it is better to take contraceptive measures in the same room.
But women are sometimes affected by the environment, emotions and fatigue, and may be ovulating early.
The following may affect the test results: menopause, taking hormones, steroids and contraceptives, or polycystic ovary syndrome, hyperthyroidism and some endocrine diseases.
6 patients with primary hypogonadism, ovarian failure, and polycystic ovary syndrome can lead to abnormal increase of LH.
Pituitary - hypothalamus, amenorrhea syndrome, Kallma syndrome, anorexia nervosa.
The lack of simple LH in pituitary, and the delay of puberty can lead to abnormal decrease of LH, so the test results should be judged in a comprehensive clinical way.
7 oviposit test paper test is not absolutely accurate, miss ovulation and LH peak of abundant clinical case, many women can only detect the weak positive (representing the ovulation or had ovulation) and detect strong positive is ovulation (representative), that's not saying no ovulation.
Pregnant mothers can also go to the house during the weak positive period [4], and the chance of successful conception is not low.
Please use under the guidance of the doctor.
In this paper, the principle of double antibody sandwich and the technique of colloidal gold labeling are adopted in the paper, which is of high accuracy in determining the time of ovulation and the "safe period" in the menstrual cycle of women.
Â
Model NO.: PTP002
Model NO.: PTP002