1. Is the background of the colloidal gold test strip deep or the gold residue running?

One of the reasons: a certain component added to the complex solution of the labeled protein is not suitable.

Solution: Modify the complex solution formulation.

2. How to remove the edge effect of the colloidal gold test strip?

One of the reasons: Gold climbs too fast or too slow.

Solution: Process the sample pad.

3. How to solve the dead gold when colloidal gold labeled protein? Or is the gold mark not on?

One of the reasons: the amount of labeled protein is insufficient.

Solution: Rescreen the appropriate amount of protein labeling.

4. What happens when the color of the colloidal gold is not normal?

One of the reasons: the container is not clean or the water used is contaminated.

Solution: The container must be clean and replace the distilled water without impurities .

5. When the colloidal gold labeled protein is lighter?

One of the reasons: the labeled protein solution contains too much salt ions.

Solution: The labeled protein solution needs to be replaced with distilled water for dialysis.

6. How to solve the false positive problem of colloidal gold test strip?

One of the reasons: the sample to be tested has many impurities and large interference.

Solution: Increase the depth of the T line according to the background of the measured sample .

7. When the colloidal gold-labeled protein is dried, the colloidal gold color becomes purple or even grayish white?

One of the reasons: the drying process has high humidity, high temperature and long time.

Solution: Control the temperature and humidity of the drying process, and the drying time of the gold bars should not exceed 5 hours .

8.   How to improve the sensitivity of the colloidal gold test strip ?

One of the reasons: an unpaired antigen was used.

Solution: Screen for paired antigens.

9. Test strip T line becomes lighter with time? Sometimes it gets darker, how to explain it?

One of the reasons: when the gold runs fast, the antigen-antibody is not firmly bound, and the color becomes lighter ; when the gold runs slowly, the antigen is resistant.

The body is firmly bonded and the color is darker .

Solution: Select the appropriate sample pad and control the climbing speed for 5-10min .

For more reasons and solutions, please click to contact us.

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