Rongcheng Jingyi Oceanic Technology Company Limited , https://www.jingyifoods.cn
challenge
Under the invitation of a large (about 7000m 3 ) biomedical R&D facility for biological purification prior to the reactivation of the facility, its facilities are widely contaminated with mouse parvovirus (MPV) and have been tried several times to solve this problem using traditional disinfection methods. . The main criteria for selecting a disinfection method are:
1) Prove effective killing of MPV;
2) Ability to deploy on a large scale to simultaneously complete biological purification of rooms, corridors and other connected areas;
3) Ability to disinfect some challenging terrain spaces such as elevators and elevator shafts;
4) Residual technology allows the facility to be put back into service soon; and
5) Provide verification documents for the bioavailability of disinfection.
Program
Bioquell's Room Biological Purification Service (RBDS) was selected to perform this task. The second floor building is divided into 4 separate areas for disinfection. To ensure efficient vapor diffusion, 17 Clarus R-type hydrogen peroxide vapor (HPV) generators were selected, and 29 Clarus R2 ventilators were used for end-of-cycle decomposition to eliminate excess HPV and placed in each zone. The test equipment monitors key parameters in real time.
Each zone is sealed prior to the start of fumigation and is maintained until the end of the HPV decomposition of the catalyst into water and oxygen. The entire process is monitored and controlled by a control computer outside the room, and a hand-held HPV sensor is used to monitor the leak around the area.
Gas biological purification cycle verification
Small stainless steel sheets sealed in "Tyvek" contain Bacillus stearothermophilus dry spores as biological indicators (BIs) to detect the effectiveness of fumigation. Two different spore numbers were used: a standard test point using a spore count of >1.0x10 6 and a challenge point using a spore count of >1.0x10 4 .
Standard BIs are distributed in the facility according to Bioquell's protocol method, and the challenge sites are pre-determined and placed in the BIs before the start of fumigation in each region, including around the site and inside the complex equipment.
result
The B. stearothermophilus indicator was recovered after completion of the ventilation phase and cultured for 7 days at 60 degrees Celsius. A positive control without fumigation will show signs of microbial growth. 600/600 BIs placed in the disinfection area were negative (two small rooms were repeatedly sterilized). BI challenge sites include: computer equipment interiors, elevator shafts, anesthesia equipment, and animal cages.
Sensitive electronic devices exposed to HPV are unaffected, showing good material compatibility for the RBDS process.
in conclusion
At the standard site 6-log kill rate, the challenge site 4-log kill rate (Bacillus stearothermophilus BI packaged in "Tyvek" material) bio-purification target is achieved throughout the facility, the entire fumigation process is Completed within six working days. The RBDS system provides a very fast and efficient fumigation system that combines rapid ventilation to produce minimal cycle times.
This system can be used in a wide range of other applications such as the elimination of specific microorganisms, routine fumigation in laboratories, including biosafety facilities, clean rooms, pharmaceutical manufacturing plants and hospitals. The RBDS system can be expanded infinitely for rapid and efficient biological purification of very large spaces and entire buildings, as in this case.
For more details on HPV bio-purification solutions including equipment and room service, please contact Bioquell China.