Cultrex® In Vitro Angiogenesis Endothelial Cell Infiltration Analysis Kit Instructions

Angiogenesis refers to the growth of new capillary blood vessels derived from existing capillaries and post-capillary venules. Endothelial angiogenesis is an extremely complex process. Usually, the neovascularization is formed by extending and expanding on the basis of the original blood vessel, and the process is similar to the typical wound healing and embryo formation process. Under the action of angiogenic factors and chemokines, vascular endothelial cells secrete proteases such as urokinase-type plasminogen activator and inhibitor, and migrate to tumor tissues through the subvascular matrix membrane. Anti-angiogenesis has become one of the important strategies for the treatment of diseases such as tumor metastasis, diabetic retinopathy, and rheumatoid arthritis.

As a global leader in cell behavioral analysis, Trevigen offers complete angiogenesis experimental solutions. Among them, the in vitro angiogenesis assay kit and the in vitro angiogenesis endothelial cell infiltration assay reagent are used to evaluate the angiogenic factors at the cellular level, the former can be used to detect endothelial cell tube formation inducers and inhibitors, the latter Accelerate screening of compounds that affect the passage of this vascular endothelial cell through the basement membrane. In addition, Trevigen's patented DIVAA product is the world's first detection system for quantitative detection of angiogenesis in vivo.

Trevigen's Cultrex® in vitro angiogenesis endothelial cell infiltration assay kit is designed to accelerate the screening of compounds that affect the vascular endothelial cells through the basement membrane process. The kit quantifies endothelial cell infiltration by chemical chemotactic agents and/or inhibitors in vitro in a flexible, standard 96-well high-throughput format. The kit uses a simplified chemotactic chamber designed with an 8 μm PET membrane; and an inhibitor control Sulforaphane, a natural cancer inhibitor used to inhibit endothelial cells in vitro. Migration/infiltration on the BME basement membrane; use of Calcein-AM in the kit for final quantitative detection of infiltrating cells.

The Trevigen Cultrex® In Vitro Angiogenesis Assay Kit provides the essential reagents for invasive assays during angiogenesis, including cell invasion assay culture chambers and membranes, extracellular matrix protein BME, coating buffer, cell eluate, Cell dissociation liquid, fluorescent dye, and inhibitor of angiogenesis are used as negative controls and the like. The product provides an efficient protocol for the screening of compounds that affect the passage of this vascular endothelial cell through the basement membrane in a 96-well format.

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Ibrutinib Intermediates

Ibrutinib is a small molecule drug that binds permanently to a protein, Bruton's tyrosine kinase (BTK) that is important in B cells; the drug is used to treat B cell cancers like mantle cell lymphoma, chronic lymphocytic leukemia, and Waldenstrom's macroglobulinemia, a form of non-Hodgkin's lymphoma.
Ibrutinib is used to treat chronic lymphocytic leukemia, Waldenstrom's macroglobulinemia, and as a second-line treatment for mantle cell lymphoma, and marginal zone lymphoma.
We produce a series of complete intermediates for Ibrutinib, intermediate I (CAS No. 330786-24-8) and intermediate II (CAS No. 143900-44-1) etc.
All of our products are with stable production and strict quality control, making sure your experience with Sunshine Biotech is always enriching, satisfying and fulfilling.