First, sterile operation 1. The inoculation room should be kept clean, scrub the countertops and walls with pulverized coal phenol soap and fumigate regularly with lactic acid or formaldehyde. Before each use, UV lamps are used to sterilize; during use, sterilizers are used to extinguish moldy bacteria, Bacillus subtilis, Salmonella and other bacteria in the air. 2. Before entering the inoculation room, the staff should do a good job of personal hygiene work. In the buffer room, work shoes, caps, work clothes and masks should be replaced. Work clothes, work shoes, and masks are only allowed in the inoculation room, and they must be replaced and disinfected regularly. 3, inoculated test tubes, flasks, etc. should be marked, indicating the name of the culture medium, bacteria, date. All items moved into the inoculation room must be wiped clean with 70% alcohol in the buffer chamber. 4, before inoculation, hands with automatic hand disinfection hand disinfection, using 75% medical alcohol as a disinfectant, the process for the induction soap machine wash - faucet wash - induction drying - inductive hand disinfection; every 60- Re-disinfect the opponent's hand for 90 minutes to block the growth and reproduction of hand bacteria. 5. The incubator and inoculation tools should be cleaned and disinfected frequently; the flame of the alcohol lamp should not leave the operation process; the tampon should not be placed indiscriminately; the inoculation tool must be sterilized before and after use. Second, air disinfection method 1. The UV disinfection method can cause the photochemical reaction of nucleic acids and enzymes in cells and kill the cells. In addition, ultraviolet rays can also cause ozone in the air to produce ozone, which has a bactericidal effect. However, the effect on fungi is poor, and it only serves as a supplementary disinfection, and it needs to be used in conjunction with drugs. Because the UV rays are harmful to the human body, do not work with the UV light turned on. 2. The ozone generator can effectively and quickly kill various microorganisms in the air and on the surface of objects. The success rate of inoculation can reach more than 97%. It is harmful to the human body. Do not work when someone is there. 3, dynamic air sterilizer, disinfection of the air inside the workshop to ensure food safety. The disinfection technology does not harm the human body when it is disinfected in the workshop, and at the same time keeps the controlled environment in the “sterile and dust-free†standard. Erythritol For Health And Personal Care Products Erythritol For Health And Personal Care Products,Aldi Erythritol,Erythritol With Monk Fruit Ningxia Eppen Biotec CO.,LTD , https://www.nxeppen.com
In the cultivation and cultivation of mushrooms, it is necessary to ensure that the strains are of good quality, to grasp and apply disinfection and sterilization techniques, and to prevent the contamination of bacteria.