Rat melatonin Elisa kit instructions l This kit is used for quantitative determination of rat melatonin in serum, plasma, tissue, cell supernatant and related liquid samples in vitro . l Validity period: 6 months l Storage conditions: 2 -8 ° C Experimental principle of rat melatonin Elisa kit The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with melatonin capture antibody, samples, standards, and HRP-labeled detection antibodies were sequentially added, incubated and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with rat melatonin in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration. Rat melatonin Elisa kit sample processing and requirements 1. Serum : Whole blood samples should be placed at room temperature for 2 hours or 4 °C overnight, centrifuged at 1000g for 20 minutes, the supernatant can be detected, or the specimens can be stored at -20 ° C or -80 ° C, but should be avoided repeatedly melt. Reagents and equipment that are needed but not provided Precautions Reagent preparation The kit should be taken out of the refrigerated environment and should be balanced at room temperature before use. Dilution of 20× Wash Buffer: Distilled water was diluted 1:20, ie 1 part of 20× Wash Buffer plus 19 parts of distilled water. Steps Experimental result calculation The OD value of the tested standard is the abscissa, the concentration value of the standard is the ordinate, the standard curve is drawn on the coordinate paper or with the relevant software, and the linear regression equation is obtained, and the OD value of the sample is substituted into the equation to calculate the sample. concentration. Kit performance Rat melatonin Elisa kit related product recommendation: Human fibroblast growth factor 8 (FGF8) ELISA kit Goat Immunoglobulin A (IgA) ELISA Kit Human heart fatty acid binding protein (h-FABP) ELISA kit Bovine serum albumin (ALB) ELISA kit Human soluble interleukin-6 (sIL-6) ELISA kit Rat transforming growth factor alpha (TGF-alpha) ELISA kit Horse vascular endothelial growth factor (VEGF) ELISA kit Rat 70kDa heat shock protein 5 (HSPA5) ELISA kit Porcine galectin-8 (GAL8) ELISA kit Guinea pig myelin basic protein (MBP) ELISA kit Rat serum ferritin (SF) ELISA kit Pig 1,25 dihydroxyvitamin D3 (1,25DHVD3) ELISA kit Rat ovarian cancer antigen (CA125) ELISA kit Human total bilirubin (TB) ELISA kit 58546-54-6 Gomisin A Human Gastrin ELISA Kit Human complement 1q (C1q) ELISA kit Human hydrogen peroxide (H2O2) ELISA kit 523-50-2 Isopsoralen Rabbit Matrix Metalloproteinase 8 (MMP-8) ELISA Kit Human dentin matrix protein 1 (DMP1) ELISA kit Rat Acetyl CoA Carboxylase (ACC) ELISA Kit Chicken carbonic anhydrase (CA) ELISA kit Rat alpha actin (α Actin) ELISA kit Rabbit monocyte chemoattractant protein 1 (MCP-1/CCL2/MCAF) ELISA kit Human TARDNA-binding protein 43 (TARDBP/TDP43) ELISA kit Human heparan sulfate proteoglycan 2 (HSPG2) ELISA kit Human immunodeficiency virus type I+II antibody ELISA test kit (qualitative) Porcine obestatin ELISA kit 611-40-5 Mouse Interleukin 10 (IL-10) ELISA Kit Mouse placental growth factor (PLGF) ELISA kit Human galanin/glycanin (GAL) ELISA kit Sheep Immunoglobulin G1 (IgG1) ELISA Kit Mouse cyclic guanosine monophosphate (cGMP) ELISA kit Mouse stem cell factor/mast cell growth factor (SCF/MGF) ELISA kit Human glucoside oleate dehydrogenase (GADPH) ELISA kit Rat melatonin Elisa kit , please contact our sales customer service hotline--021-60520062, 18721729907 QQ, welcome your inquiry, request for instructions, inquiry message form, telephone, online customer service QQ, send email can. Keshun Bio is a professional high-quality Elisa kit, production, supplier, we will be sincere, please rest assured to buy! Note: All products of the company are for scientific research only and should not be used for clinical purposes.
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2. Plasma : EDTA or heparin can be used as anticoagulant. The specimen should be centrifuged at 2-8 °C for 1000 minutes within 20 minutes after collection, or the specimen should be stored at -20 °C or -80 °C, but repeated freezing and thawing should be avoided. .
3. Cell culture supernatant or other biological specimens : centrifuge at 1000g for 20 minutes, take the supernatant to detect, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing and thawing.
Note: Specimen hemolysis will affect the final test results, so hemolysis specimens should not be tested.