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HPLC deuterium lamp - Analysis on Causes baseline instability issues 
The technical performance indicators of xenon lamps usually include three important indicators: xenon lamp energy, noise, and drift. Among them, for the end user, the most likely problems are concentrated on the xenon lamp energy, but in the xenon lamp During the sales process, customers who often purchased xenon lamps often reported that the xenon lamp baseline was unstable. Later, through repeated confirmation and comparison, it was found that in most cases, the problem was not a xenon lamp. Therefore, in the following In the content, we have made a simple analysis and discussion on several situations in which the frequent occurrence of unstable baseline drift is serious, and I hope to be instructive to everyone.
Situation 1:
In the course of a day's work, the baseline has been very stable, but after a long period of time, the baseline drift will be severe, as shown in Figure a.
Figure a
Reason analysis: Most of them are mainly related to sudden changes in temperature, and the temperature difference is abrupt.
For example, during the daytime, the laboratory temperature is moderate, and the weather temperature drops sharply at night. At this time, the above situation will occur, the baseline suddenly drifts seriously, or the reagents are more improved. The temperature of the new injection reagent is very low (no column temperature is provided). Box), there will also be a baseline drift.
Case 2:
The baseline has been very stable all the time, but the baseline jumps suddenly, and the baseline is stable at the new position after the beating, as shown in Figure b.
Figure b
Reason analysis: Most of them are mainly caused by the instability of the instrument.
For example, the pressure of the pump of the instrument suddenly increases, or the instrument is suddenly subjected to physical vibration during use. At this time, the above situation occurs, and the baseline suddenly jumps, but there is no serious phenomenon of baseline drift.
Situation 3:
The baseline drift was severe at first, but the baseline stabilized after a while, as shown in Figure c.
Figure c
Reason analysis: Most of them are mainly related to the new lamp.
After the replacement of the new xenon lamp, it takes about 48 hours to reach a stable state. Therefore, it is better to test it after 48 hours of lighting after replacing the new lamp.
Situation 4:
The drift of the baseline interval is regular, the time interval is the same, as shown in Figure d
Figure d
Reason analysis: Most of them are mainly related to the pump and column of the instrument.
For example, the intermittent operation of the pump or column spacing results in a spacer drift of the baseline.
Case 5:
The irregular spacing of the baseline produces drift, as shown in Figure e.
Figure e
Reason analysis: Most of them are mainly related to the sample liquid.
For example, the injection solution contains bubbles and the like.
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