Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is reduced by NAD+ plus H, and NAD+ is oxidized by NADH. In the 2' position of adenine nucleotides, an ester bond is added through an ester bond to form a phosphate group. NAD/NADH Quantitative and Ratio Analysis Kit - Coenzyme NAD (NADH) Research Protocol NADP. NAD or NADP as a coenzyme participates in the redox reaction and electron transfer essential for normal cell life.

The NAD/NADH ratio in a normal organism is always in a state of dynamic equilibrium. This ratio is a very important indicator that reflects the redox state of a cell, thereby determining the metabolic activity of a cell and the health of the cell. In healthy mammalian tissues, the ratio of NAD/NADH is approximately 700. As a technology company dedicated to providing high-end products, technical support and services to high-end customers such as scientific research institutions and universities, Amy Jet introduces you to the NAD/NADH ratio analysis of the hotspots of cell metabolism research, including convenient Different complete research protocols, such as colorimetric detection and more sensitive fluorescence detection, to meet the experimental conditions of your different customers.

The Amplite Fluorimetric NAD/NADH Ratio Assay Kit (Cat. No. AAT-15263) is a fluorescent detection NAD/NADH ratio detection kit with high sensitivity. This detection method can significantly reduce interference from the biological sample itself because it is in the red visible light range. The detection method exhibits high sensitivity and small interference, is excited at 570 nm, emits light at 590 nm, and can detect NAD, NADH, and NAD/NADH ratios, respectively. The NAD/NADH Quantitation Colorimetric Kit (Cat. No. K337-100) is a colorimetric test kit that is easy to use in most laboratories and is easy and fast to operate. The above two kits recommended for you can detect NAD, NADH and NAD/NADH ratio data, with unbeatable price/performance ratio and absolute value! The experiment is simple, convenient, sensitive, and highly specific, and does not cross-react with NADP.

NAD/NADH定量与比率分析试剂盒—辅酶NAD(NADH)研究方案

If you want to perform high-throughput analysis of more samples, or if you want to test directly on a 96-well cell culture plate, the NAD+/NADH Cell-Based Assay Kit (Cat. No. 600480-1) is the right choice. The kit is to culture cells in a 96-well plate, complete a set of experiments in a 96-well plate based on colorimetric method, and finally obtain the ratio of NAD/NADH. The entire experiment is simple to operate and is especially suitable for high-throughput studies. Slightly regrettable, the kit can only detect NAD/NADH ratios. In addition, Amy Jie also recommends a test kit for detecting NADH alone and detecting total NAD/NADH. It is also suitable for colorimetric and fluorescent methods, so that you can choose the right test solution according to your needs.

In addition to the NAD/NADH Quantitative and Ratio Analysis Kit, Amy Jie recommends other commercial kits, including NADP/NADPH quantification and ratio analysis, as well as other glucose metabolism, glycolysis pathways, pyruvate metabolism. Analytical kits for other cellular metabolism such as Lactic Acid metabolism, to help customers with scientific research!

Amy Jie can find and provide the best product solutions for you! Including a variety of detection and analysis kits, related antibodies, recombinant proteins and cytokines and enzymes, as well as other innovative research tools. Its products cover areas such as apoptosis research, energy metabolism, cell/tissue protein extraction, oxidative stress and injury, metabolism and obesity, cell proliferation and toxicity, signal transduction, and stem cell research. Amy is committed to providing the best and most up-to-date research tools in cell biology to continuously expand the quality of its products and services. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions. Article source: http://?post=1409&action=edit

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