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New method for detecting bacteria: Apogee flow cytometry
Simple, fast and accurate
Foodborne diseases are mainly caused by pathogenic bacteria through food, and these diseases have been on the rise in recent years. The general food will be sterilized by different disinfection methods, and the food factory will once again determine whether the efficiency of sterilization is sufficient. The current method of detecting bactericidal efficiency is to measure the number of colonies of the food sample on the medium. The method is to grow, replicate and eventually form colonies on the culture medium in the sample. However, this method ignores bacteria that are still alive but cannot be cultured in a laboratory environment, and these bacteria also have the opportunity to cause disease. So microbiologist start looking for some new technology to detect biological, hoping to find some quick and accurate way.
original
April this year, Italian scientists in the academic journal Microbiology published a scholarly article entitled "Comparison of quantitative PCR and flow cytometry as cellular viability methods to study bacterial membrane permeabilization following supercritical CO2 treatment" (159,1056-1066) of. The study used the recently emerging supercritical carbon dioxide sterilization (SC-CO2) to treat Listeria, Salmonella and Escherichia coli, and then used real-time PCR and Apogee flow cytometry techniques to compare sterilization with conventional colonies. It is concluded that the flow detection technique is more accurate and comprehensive than other methods, and it can clearly analyze intact cells, permeabilized cells and semi-permeable cells in the sample.
For details, please see: http://mic.sgmjournals.org/content/159/Pt_6/1056.short
The flow cytometer from Apogee, UK, has excellent performance and can detect microparticles as small as 100 nm, so it is often used in special fields such as microbiology and bacterial detection.
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