For those who use optical microscopes for the first time, the magnification of the microscope may be more confusing, how is the total magnification calculated, and how many times the photographs are taken. There are two concepts of total magnification, one is optical magnification, and the other is digital magnification (digital magnification is only involved when connected to an imaging device).
1. Optical magnification. It refers to the multiples we have observed from the microscope eyepiece after the object has been enlarged. The calculation method of the optical magnification is relatively simple, that is, the objective lens multiple * eyepiece multiple. For example, the magnification of a stereo microscope is usually 0.7-4.5 times that of a continuous zoom stereo microscope, and in the case of a 10x eyepiece, the total magnification of the microscope is 7-45 times; The calculation of the metallographic microscope is simpler. The general objective lens configuration is 4 times, 10 times, 40 times, 100 times. The conventional configuration of the eyepiece is 10 times, and there are 16 times, 20 times, etc., as long as the eyepiece and objective lens are The multiples are multiplied to obtain the total magnification.
2. Digital magnification. Digital amplification refers to the magnification of the image displayed on the external device. At present, more on the market is a three-eye microscope connected to a computer, monitor or television through CCD equipment for imaging observation to reduce eye fatigue. It is also easy to share with others. But how many times does the object displayed on the image zoom in? I recommend two methods for calculating digital amplification.
(1) Directly measure the image. Place the micrometer under the microscope, then directly measure the length of the micrometer on the display with a ruler, and measure the actual length of each cell on the display/the micrometer (usually on the micrometer) There is a length of each grid) = multiples of the object being magnified. The multiple of the object being magnified / the multiple of the current objective lens = digital magnification. Normally, a scale is added to the image to indicate the multiple of the object being magnified.
Note: If there is no micrometer, you can use a ruler instead, and you can measure a few more in the calculation to reduce the error.
(2) Calculate the actual magnification by the formula.
Digital Magnification = Objective Multiplier *{25.4* Screen Size (inches) / CCD Diagonal Length} * Adapter Magnification, if system magnification, multiply by system magnification.
Note:
1: The objective lens multiple refers to the multiple of the objective lens of the microscope you are using now, such as 20 times;
2: Magnification of the adapter: refers to the magnification of the connection between the microscope and the imaging device, usually 1 times, also 0.35, 0.5, 0.63 times;
3:25.4* screen size (inches): here is the screen size converted to millimeters, 1 inch = 25.4mm;
4: CCD diagonal length: refers to the CCD chip size, often 1/3 inch, 1/2 inch, 2 / 3 inch, the corresponding length is 6mm; 8mm; 11mm, this is the industry Uniform and standardized.

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